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Georgia's Metropolitan Atlanta rapidly Transit Authority uses IBM Watson IoT apparatus to support predict, identify and restore abilities issues with censorious device and machinery
ARMONK, N.Y., Feb. 28, 2019 /PRNewswire/ -- IBM (NYSE: IBM) these days announced a brand recent portfolio of web of issues (IoT) solutions that team synthetic intelligence (AI) and superior analytics to allay asset intensive corporations, such because the Metropolitan Atlanta quick Transit Authority (MARTA), to augment renovation concepts. The solution is designed to support companies to lessen expenses and in the reduction of the possibility of failure from actual property equivalent to motors, manufacturing robots, turbines, mining machine, elevators, and electrical transformers. IBM Maximo Asset efficiency management (APM) options assemble statistics from physical belongings in near precise-time and supply insights on existing operating conditions, prognosticate talents concerns, determine complications and offer restoration recommendations.
IBM)" alt="IBM these days introduced a recent portfolio of web of things options, IBM Maximo Asset performance administration, bringing together synthetic intelligence and advanced analytics to assist asset intensive organizations, such as the Metropolitan Atlanta rapid Transit Authority (MARTA) in Georgia, to enhance preservation innovations. The solution collects records in close real-time from any physical asset, reminiscent of vehicles, manufacturing robots, mills, mining machine, elevators, electrical transformers and extra, an d provide insights on present working situations, predicts competencies considerations, identifies complications and presents restore techniques. (IBM)" />
businesses in asset-intensive industries like energy and utilities, chemical compounds, oil and gas, manufacturing, and transportation, can abide thousands of belongings that are principal to operations. These assets are more and more producing large quantities of information on their working circumstances. holding these assets up and running is essential to operations and monitoring and optimizing property on-going maintenance, fix, and substitute selections requires regular evaluation. in response to analyst firm Aberdeen research, unplanned downtime can can permeate a corporation as much as $260,000 an hour.
company leaders can now recognize if a laptop is probably going to fail, the exhaust of advanced analytics and AI to raise into account timing, prognosticate consequences and establish issues. IBM's APM solutions support companies shift asset renovation thoughts from preventative to predictive and prescriptive with the aid of integrating disparate information sources to ascertain property in need of attention and recommending actions.
This reply enhances a corporation's current traffic asset management (EAM) capabilities, reminiscent of IBM's market leading Maximo EAM solution, and integrates seamlessly with other EAM suppliers. It contains:
IBM will additionally present the APM suite customized for specific industries, dawn with APM for power and Utilities (E&U). This provides business-selected capabilities to research and act on insights from utility belongings and contains risk/criticality scoring, health and degradation fashions, common traffic information mannequin, and weather facts integration.
"it's essential for businesses to mediate about how helpful their renovation practices are. With the launch of its recent Asset efficiency management solutions, IBM is assisting companies Make perception-pushed selections with an integrated portfolio of superior analytics and AI capabilities to assist them enrich operational effectiveness and efficiency," pointed out Kareem Yusuf, Ph.D., universal manager, IBM Watson IoT. "Maximo APM is designed to enhance a firm's existing enterprise Asset administration gadget by pass of addressing every bit of points of asset performance together with fitness, predictive maintenance and restore optimization."
MARTA keeps Atlanta, Georgia running
The Metropolitan Atlanta speedy Transit Authority (MARTA), the foremost public transit agency in the Atlanta metropolitan enviornment, is working with IBM to assign in obligate a predictive preservation reply to enhance reliability of property, minimize expenses and create a Transit Asset management (TAM) device that offers asset inventory, situation assessment, efficiency measures and election support. through data mining, computer researching and AI, MARTA can entry and anatomize statistics to enhanced recall the circumstance of machine classified in the classes of life defense, operation censorious and operation support to determine potential issues of a "gadget" with assorted stakeholders. subsequently, the reply will allow MARTA to seamlessly circulation from monitoring asset efficiency KPIs to predicting and fighting asset failures.
"MARTA is on course to swirl into the primary North American public transit company to obtain ISO 55000 certification. taking fragment with IBM gives MARTA with the innovation from a expertise icon, which fortifies us as an industry chief in Transit Asset management," talked about Remy Saintil, director of facilities at MARTA.
About IBMIBM options are designed to allow customers to augment the operational efficiency of their physical belongings and reduce fees during the power of IoT information and synthetic intelligence. by using amassing and curating statistics from billions of related contraptions, sensors, and methods international, they empower companies to derive recent insights from their equipment, automobiles and amenities. IBM's trade knowledge, leading protection, and options constructed for a hybrid cloud atmosphere firmly establish us as a leader in IoT. For extra assistance on IBM Watson IoT, delight talk over with www.ibm.com/iot.
IBM corporation emblem. (PRNewsfoto/IBM)greater
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February 18, 2019 Timothy Prickett Morgan
well, that took a little a long time. After what seems like eons of nudging and cajoling and pushing, IBM is making the IBM i operating apparatus and its built-in database administration gadget, as well as the application construction apparatus and other programs application, attainable on its self-branded IBM Cloud public cloud.
massive Blue previewed its plans to bring each IBM i and AIX to the IBM Cloud at its annual suppose conference in Las Vegas, on scale out machines aimed toward small and medium corporations as well as to clients who want to dash clusters of machines, and on scale up programs that abide NUMA electronics that more tightly cluster them into shared reminiscence techniques.
There are a lot of questions about how this might subsist every bit of subsist packaged up and bought under the unwieldy cognomen of the IBM vigour methods digital Server on IBM Cloud. however they are able to let you know every bit of that they understand and fill you in as they gain knowledge of greater.
The vim programs slices served up on the IBM Cloud operating IBM i and AIX abide been first rolled out for early entry to chosen valued clientele to check the APIs for the handle plane, which they presume is in keeping with the PowerVC implementation of OpenStack. The prefatory customers who're kicking the tires had been supposed to simplest installation development and test environments or protype code, not at every bit of the rest working in production. This was absolutely supposed to subsist a proof of concept setup at this point, as you could contemplate from the rollout roadmap:
The alpha and beta trying out for the energy programs slices on the IBM Cloud is on a honorable looking quickly music, operating from February into March, as which you could see, and commonplace availability is slated for a while in March. doubtless somewhere towards the end, they would wager. IBM is at the dawn making the power puffs – what else are you able to cognomen them as shorthand? – obtainable in the early entry exams in its US East datacenter in the Washington DC metro area; it isn't limpid what iron turned into used. The alpha and beta testing is being completed on a group of vigour S922 scale out machines, which can subsist in response to a pair of Power9 processors and which frankly is a large box for many IBM i stores every bit of with the aid of itself, as well as on energy E880 scale up methods, that are being scaled as much as sixteen sockets and which exhaust the older Power8 processors. every bit of of the machines exhaust ten-core processors, which are the sweet spot between efficiency, chip yield, and charge within the energy techniques line. The Power9 chips within the energy S922 servers dash at 2.8 GHz and might turbo as much as three.9 GHz if there is satisfactory thermal headroom; these chips emerge to subsist they may abide four threads per core (often known as SMT4), which isn't as a gross lot threading as the ordinary Power8 chip offers in scale out machines with SMT8 threading (eight threads per core) and twice as many cores per die (24 cores versus 12 cores). Anyway, it seems like the power S922 is being equipped with the heavy cores, now not the mild ones. The Power8 chips within the power E880 are operating at 4.19 GHz and additionally steer SMT8 threading (there was only a heavy core privilege here); every bit of sixteen cores are populated with ten-core Power8 chips, with a maximum scalability of 160 cores in a solitary ratiocinative partition on the IBM Cloud.
each of the machines are being configured with 32 GB reminiscence sticks, which can subsist once again at the sweet spot between means, bandwidth, and charge per bit, and IBM is allowing valued clientele to configure the machines with anywhere from 8 GB to 64 GB of leading reminiscence per core. if you need denser reminiscence to bolster the skill, that you would subsist able to double it up the exhaust of sixty four GB reminiscence sticks, and then double it up again to 128 GB sticks, however this may subsist a custom order and it will can permeate significantly extra, they consider. IBM is providing disk and scintillate storage as much as 2 TB for autochthonous storage on the situations and growing to subsist at 10 GB increments. external storage for applications and databases is on Storwize V7000 disk arrays in the racks close this vigour iron, and are linked to the servers via Fibre Channel switches. valued clientele can share machines or employ dedicated iron, reckoning on their proclivities for absolute security and coping with strident neighbors on the digital infrastructure.
Steve Sibley vp and offering supervisor for the Cognitive programs division at IBM, gave me a sneak peek at the future energy cloud slices on a talk over with to the Austin, Texas labs final week, forward of the mediate convention. here is how the facets and functions of the ratiocinative partitions on the IBM Cloud are shaping up:
Sibley told us that the pricing changed into now not yet set in stone for the digital energy capacity on the IBM Cloud, but when pressed about evaluating the cost between buying apparatus and renting it on the cloud, Sibley estimated that for a given IBM i or AIX workload running on in a similar pass configured hardware and ratiocinative partitions, running the cloud instance would permeate about 30 p.c to 40 p.c extra on the illustration working replete out over three years compared to buying the iron and the exhaust of it for three years. The difference, of direction, is that IBM is managing the vim systems and purchasing the datacenter, the electricity, the cooling, the security, patch updates on firmware, the installing of the PowerVM hypervisor and its replace, and the setting up of the IBM i or AIX working device. When organizations purchase their own energy systems machines, until they accept augmented features from IBM or a enterprise companion, it’s every bit of on them. Pricing for the digital machines, the reminiscence, the storage, and the working apparatus and databases are every bit of set per hour.
incidentally, it isn't limpid what IBM i and AIX releases are supported on the machines, but most likely the existing IBM i 7.2 and IBM i 7.three releases are supported on the IBM Cloud as well as AIX 7.1 and AIX 7.2, that are likewise present. Linux isn't mentioned as a supported platform, but SAP HANA workloads are and that means that there is a Linux – and above every bit of red Hat enterprise Linux – beneath it considering that HANA in-reminiscence databases are not supported on any Unix variant, or IBM i for that remember.
by means of March, IBM plans to abide the IBM i and AIX instances purchasable in creation in its US East (Washington DC) and US South (Dallas) regions, and in the 2d quarter IBM plans to roll it in its European datacenter in its eu imperative (Frankfurt) area, with different datacenters coming out as demand pulls and IBM pushes.
we are able to raise a glance on the pricing as soon because it turns into obtainable and spot how it every bit of stacks up, and accept the suggestions of alternative IBM i cloud avid gamers about what huge Blue is doing.
Public Cloud Dreaming For IBM i
Skytap Says It’s pile a ‘authentic Cloud’ offering for IBM i
a far better approach to skin The IBM i Cloud Cat
What Cloud providers should accomplish To entice IBM i
Google near Launching IBM i Cloud service, Will Says
Is The ‘Golden Age’ of Computing Leaving IBM i at the back of?\
Ensono Emerges With IBM i Cloud providing
Clouds grow, however Can IBM i observe?
IBM ultimately receives Power8 Machines On SoftLayer Cloud
All signals point To The Cloud, however Will IBM i crowd comply with?
mobile Apps And The IBM i concern manufacturing unit
Infor Readies IBM i Cloud
IBM i Finds a situation in the Cloud
vigour programs Coming To The SoftLayer Cloud
IBM Brags About Its Cloud Prowess due to SoftLayer
IBM Buys SoftLayer To build Out hosting, Cloud agencies
Two query TPM Questions: precise IBM i Clouds And Apple buying IBM i
IBM i 7.1 Tech Refresh activities live Partition Mobility
AWS/four hundred: Amazon Builds An AS/four hundred-oid Cloud
wanted: Cloud-i i-nfrastructure
The X factor: Head within the Clouds
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Feb 28, 2019 (Heraldkeeper via COMTEX) -- Global Airport Digitization Industry
New Study On "2018-2025 Airport Digitization Market Global Key Player, Demand, Growth, Opportunities and Analysis Forecast" Added to sensible Guy Reports Database
This report focuses on the global Airport Digitization status, future forecast, growth opportunity, key market and key players. The study objectives are to present the Airport Digitization development in United States, Europe and China.
In 2017, the global Airport Digitization market size was million US$ and it is expected to gain million US$ by the discontinuance of 2025, with a CAGR of during 2018-2025.
The key players covered in this studyCisco SystemsIBMMicrosoftSiemensSITAAppleScarabeeWind RiverDaifukuLiving PlanIT
Try Sample Report @https://www.wiseguyreports.com/sample-request/3552470-global-airport-digitization-market-size-status-and-forecast-2018-2025
Market segment by Type, the product can subsist split intoHardwareSoftwareService
Market segment by Application, split intoPassenger Screening and SecurityBaggage ServicesPassenger AssistanceOther
Market segment by Regions/Countries, this report coversUnited StatesEuropeChinaJapanSoutheast AsiaIndiaCentral & South America
The study objectives of this report are:To anatomize global Airport Digitization status, future forecast, growth opportunity, key market and key players.To present the Airport Digitization development in United States, Europe and China.To strategically profile the key players and comprehensively anatomize their development draw and strategies.To define, report and forecast the market by product type, market and key regions.
For circumstantial Reading delight visit WiseGuy Reports @ https://www.wiseguyreports.com/reports/3552470-global-airport-digitization-market-size-status-and-forecast-2018-2025
Some Major Points from Table of content:
1 Report Overview1.1 Study Scope1.2 Key Market Segments1.3 Players Covered1.4 Market Analysis by Type1.4.1 Global Airport Digitization Market Size Growth Rate by ilk (2013-2025)1.4.2 Hardware1.4.3 Software1.4.4 Service1.5 Market by Application1.5.1 Global Airport Digitization Market share by Application (2013-2025)1.5.2 Passenger Screening and Security1.5.3 Baggage Services1.5.4 Passenger Assistance1.5.5 Other1.6 Study Objectives1.7 Years Considered
2 Global Growth Trends2.1 Airport Digitization Market Size2.2 Airport Digitization Growth Trends by Regions2.2.1 Airport Digitization Market Size by Regions (2013-2025)2.2.2 Airport Digitization Market share by Regions (2013-2018)2.3 Industry Trends2.3.1 Market Top Trends2.3.2 Market Drivers2.3.3 Market Opportunities
3 Market share by Key Players3.1 Airport Digitization Market Size by Manufacturers3.1.1 Global Airport Digitization Revenue by Manufacturers (2013-2018)3.1.2 Global Airport Digitization Revenue Market share by Manufacturers (2013-2018)3.1.3 Global Airport Digitization Market Concentration Ratio (CR5 and HHI)3.2 Airport Digitization Key Players Head office and district Served3.3 Key Players Airport Digitization Product/Solution/Service3.4 Date of Enter into Airport Digitization Market3.5 Mergers & Acquisitions, Expansion Plans
4 Breakdown Data by ilk and Application4.1 Global Airport Digitization Market Size by ilk (2013-2018)4.2 Global Airport Digitization Market Size by Application (2013-2018)
5 United States5.1 United States Airport Digitization Market Size (2013-2018)5.2 Airport Digitization Key Players in United States5.3 United States Airport Digitization Market Size by Type5.4 United States Airport Digitization Market Size by Application
6 Europe6.1 Europe Airport Digitization Market Size (2013-2018)6.2 Airport Digitization Key Players in Europe6.3 Europe Airport Digitization Market Size by Type6.4 Europe Airport Digitization Market Size by Application
7 China7.1 China Airport Digitization Market Size (2013-2018)7.2 Airport Digitization Key Players in China7.3 China Airport Digitization Market Size by Type7.4 China Airport Digitization Market Size by Application
8 Japan8.1 Japan Airport Digitization Market Size (2013-2018)8.2 Airport Digitization Key Players in Japan8.3 Japan Airport Digitization Market Size by Type8.4 Japan Airport Digitization Market Size by Application
9 Southeast Asia9.1 Southeast Asia Airport Digitization Market Size (2013-2018)9.2 Airport Digitization Key Players in Southeast Asia9.3 Southeast Asia Airport Digitization Market Size by Type9.4 Southeast Asia Airport Digitization Market Size by Application
10 India10.1 India Airport Digitization Market Size (2013-2018)10.2 Airport Digitization Key Players in India10.3 India Airport Digitization Market Size by Type10.4 India Airport Digitization Market Size by Application
11 Central & South America11.1 Central & South America Airport Digitization Market Size (2013-2018)11.2 Airport Digitization Key Players in Central & South America11.3 Central & South America Airport Digitization Market Size by Type11.4 Central & South America Airport Digitization Market Size by Application
12 International Players Profiles12.1 Cisco Systems12.1.1 Cisco Systems Company Details12.1.2 Company Description and traffic Overview12.1.3 Airport Digitization Introduction12.1.4 Cisco Systems Revenue in Airport Digitization traffic (2013-2018)12.1.5 Cisco Systems Recent Development12.2 IBM12.2.1 IBM Company Details12.2.2 Company Description and traffic Overview12.2.3 Airport Digitization Introduction12.2.4 IBM Revenue in Airport Digitization traffic (2013-2018)12.2.5 IBM Recent Development12.3 Microsoft12.3.1 Microsoft Company Details12.3.2 Company Description and traffic Overview12.3.3 Airport Digitization Introduction12.3.4 Microsoft Revenue in Airport Digitization traffic (2013-2018)12.3.5 Microsoft Recent Development12.4 Siemens12.4.1 Siemens Company Details12.4.2 Company Description and traffic Overview12.4.3 Airport Digitization Introduction12.4.4 Siemens Revenue in Airport Digitization traffic (2013-2018)12.4.5 Siemens Recent Development12.5 SITA12.5.1 SITA Company Details12.5.2 Company Description and traffic Overview12.5.3 Airport Digitization Introduction12.5.4 SITA Revenue in Airport Digitization traffic (2013-2018)12.5.5 SITA Recent Development12.6 Apple12.6.1 Apple Company Details12.6.2 Company Description and traffic Overview12.6.3 Airport Digitization Introduction12.6.4 Apple Revenue in Airport Digitization traffic (2013-2018)12.6.5 Apple Recent Development12.7 Scarabee12.7.1 Scarabee Company Details12.7.2 Company Description and traffic Overview12.7.3 Airport Digitization Introduction12.7.4 Scarabee Revenue in Airport Digitization traffic (2013-2018)12.7.5 Scarabee Recent Development12.8 Wind River12.8.1 Wind River Company Details12.8.2 Company Description and traffic Overview12.8.3 Airport Digitization Introduction12.8.4 Wind River Revenue in Airport Digitization traffic (2013-2018)12.8.5 Wind River Recent Development12.9 Daifuku12.9.1 Daifuku Company Details12.9.2 Company Description and traffic Overview12.9.3 Airport Digitization Introduction12.9.4 Daifuku Revenue in Airport Digitization traffic (2013-2018)12.9.5 Daifuku Recent Development12.10 animate PlanIT12.10.1 animate PlanIT Company Details12.10.2 Company Description and traffic Overview12.10.3 Airport Digitization Introduction12.10.4 animate PlanIT Revenue in Airport Digitization traffic (2013-2018)12.10.5 animate PlanIT Recent Development
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Figure 2 shows a schematic representation of the study design. Sixteen hardy virile BALB/c mice were assigned to one of four radiopharmaceutical groups. Intraperitoneal (i.p.) administration of 15 mg/kg DOX was applied in every bit of groups every three weeks for one, two, three, or four cycles, respectively. The maximum cumulative dose comprised 60 mg/kg given over 12 weeks, which corresponds to a potentially cardiotoxic dose of 1050 mg per cycle for a 70-kg virile patient10. At baseline and after completing each cycle of DOX, the cardiac and other organ biodistribution of the four radiopharmaceuticals was investigated approximately one hour after intravenous (i.v.) tail vein administration. After a group of four mice completed the regimen, the left ventricular (LV) duty was evaluated by transthoracic echocardiography. Then animals were sacrificed for non-imaging assessment, which included deoxynucleotidyl transferase biotin-dUTP nick-end labeling (TUNEL) staining, cardiac expression of Bcl-2, p53, caspase 3, caspase 8, and HIF-1α and Δψm by Western blot (WB) analysis, immunohistochemistry (IHC), and fluorescence-activated cell sorting (FACS)/flow cytometry. Furthermore, a group of four hardy BALB/c mice per radiopharmaceutical served as controls, as they were treated at the identical time-points and conditions with saline administration. They were likewise imaged after every cycle, but were only sacrificed after completing every bit of four saline cycles. The study was approved by the animal supervision committee of the University of Pisa in accordance with material animal supervision guidelines.Figure 2
Schematic study design. Non-imaging markers are WB, IHC, and FACS expression markers. † = sacrifice of mice.Radiopharmaceutical preparation and administration 99mTc-sestamibi
The lipophilic cation Sestamibi (Cardiolite, Bristol-Myers Squibb) was radiolabeled by adding water to 1–3 ml of sodium pertechnetate (99mTcO4)Na and heated for ten minutes at 100 °C. After cooling, a monovalent lipophilic cationic involved was retrieved (99mTc-sestamibi-6–2-metossi-isobutyl-isonitril), of which an incurious of 18.5 MBq in 0.1 mL was injected i.v.99mTc-Annexin V
Human recombinant Annexin V (National Institutes of Health, Bethesda, MD, USA) was radiolabeled through combined in vitro incubation at elbowroom temperature for 30 minutes of 60–90 μg di-HYNIC-Annexin V diluted in 0.5 ml of physiological saline solution, 0.74–1.11 MBq pertechnetate anion (99mTcO4−), which was freshly eluted by a 6 GBq generator (Amersham, GE HealthCare), 10–20 μg of reduction agent (stannous chloride dehydrate, SnCl2−2H2O), and 1.5 mg of tricin (N-[tri(hydroxymethyl)methyl]glycine) as co-ligand. Labeling submit was 85–90%. 99mTc-Annexin V was then injected in a 0.1 mL solution containing on incurious 18.5 MBq of 99mTc-Annexin V, which corresponds to a total protein concentration of 4.5 ng per animal.99mTc-glucaric acid
99mTc-glucaric acid (Molecular Targeting Technologies, Inc. West Chester, PA, USA) was prepared from a lyophilized kit containing 12.5 mg of D-glucaric acid and 0.15 mg of stannous chloride11 by adding 1480 MBq (40 mCi) freshly eluted 99mTc-pertechnetate as previously described12. Labeling efficiency (always >98%) was checked by ascending chromatography. The radiopharmaceutical was injected in 0.1 mL solution, containing on incurious 18.5 MBq 99mTc-glucaric acid.[18F]FDG
The glucose analogue [18F]FDG was provided as a bulk vial (Gluscan, GE Healthcare), from which a sample of 18.5 MBq in 0.1 ml was injected. To avoid the intake of carbohydrates, the mice that received [18F]FDG were provided a fat-enriched diet.Echocardiographic evaluation
LV duty was evaluated with transthoracic echocardiography before the mice were euthanized, approximately 10 minutes after initiation of sedation. M-mode images were used for measurements of LV end-diastolic internal diameter (LVEDD) and LV end-systolic internal diameter (LVESD) in two consecutive beats. Fractional shortening (FS) was calculated as FS% = [(LVEDD − LVESD)/LVEDD] × 100.Biodistribution and histological analysis
After completion of their regimen and one hour after injection of the radiopharmaceuticals, animals were anesthetized and sacrificed. For biodistribution studies, samples of thyroid, kidney, tail, bone, lung, heart, muscle, blood, stomach, skin, liver, spleen, intestine, and urine were removed and weighed immediately. Radioactivity of these samples was registered for 180 seconds by a gamma counter (2470 Automatic Gamma Counter Wizard, PerkinElmer, Waltham, MA, USA), along with an aliquot of the injective and the tail to redress for partial extravasation, expressed as a percentage of the injected dose per gram of tissue (%ID/g).
Before gamma counting, two three-millimeter cardiac tissue slices were obtained for histology: one was frozen at −80 °C in desiccate ice and the other was placed in 4% formalin. After treatment with a protease inhibitor (1:100 in 10 mM Tris buffer, pH 8), apoptotic cells were assessed with direct fluorescence fragment discontinuance labeling of DNA breaks (TUNEL kit, Fluorescein FragEL, Calbiochem, San Diego, CA, USA). Caspase 3 and HIF-1α were immunostained using antihuman-mouse energetic antibodies (rabbit anti-caspase 3 and rabbit anti-HIF polyclonal antibody, Chemicon International, Temecula, CA, USA) after incubation with a fluorescent-labeled secondary antibody (Alexa Fluor 488 goat anti-rabbit IGg, Thermo Fisher Scientific, Waltham, MA, USA). Cells stained with TUNEL, caspase 3, and HIF-1α were counted in ten randomly selected high-power tumor fields (magnification x200) and expressed as a percentage of total cells. Furthermore, cardiac samples were analyzed by Western blotting to identify the specific amount of Bcl-2, p53 (both Santa Cruz Biotechnology Inc., Dallas, TX, USA), caspase 3, and caspase 8 (both Cell Signaling Technologies Inc., Danvers, MA, USA).Measurement of Δψm
Inner Δψm was measured by a solitary fluorescent lipophilic cation 5,5′,6,6′-tetrachloro- 1,1′,3,3′-tetraethylbenzimidazolocarbocyanine iodide (JC-1) that marks the loss of potential. For this purpose, mitochondria were isolated from myocardial tissue using a dedicated kit (Mytocondria Isolation Kit, Sigma-Aldrich, St. Louis, MO, USA) stained with JC-1 and analyzed by FACS (MACSQuant, Miltenyi Biotec, Bergisch Gladbach, Germany). JC-1, concentrated in the mitochondrial matrix of proper cells due to the electrochemical potential gradient, forms red fluorescent aggregates. Any event that dissipates the mitochondrial membrane potential prevents the accumulation of the JC-1 dye in the mitochondria. Accordingly, red stained cells portray intact mitochondria, whereas when the dye is dispersed throughout the entire cell, a shift from red (J-aggregates) to green fluorescence (JC-1 monomers) is observed. A red-to-green (i.e., intact to disrupt mitochondrial) ratio was performed and described as upper and lower margin (UR and LR). Consequently, a decreasing ratio was considered a disintegration of membrane potential.Statistical analysis
Biodistribution and immunofluorescent data are expressed as subsist substantive and median %ID/g tissue and standard divergence (SD). Data were analyzed by non-parametrical tests (Kruskal-Wallis and Dunn) to evaluate the differences in groups at different time points and by Mann-Whitney U to test the significance between DOX-treated and DOX-naïve mice. Furthermore, Spearman’s rho was used to assess correlations. Significance was set at p < 0.05. Statistics were performed using IBM SPSS 20 for Windows. The datasets generated and/or analyzed as fragment of the current study are available from the corresponding author by reasonable request.
ContractingGSA mulls recompete of SAM.gov contract
The generic Services Administration granted IBM a six-month $16 million extension on its condense to operate and modernize the SAM.gov system as the agency considers whether to assign the traffic up for bidding.
The extension was announced in a contracting document dated Aug. 13 that cited an pressing need to support IBM on the job while the agency explores the possibility of a competitive offering.
IBM has held the System for Award Management Architecture and Operations condense Services condense for eight years, and it is leading the effort to modernize the system of systems that government agencies and vendors exhaust to manage hundreds of billions in government contracts annually.
IBM's condense expired Aug. 14. GSA had intended to award IBM another long-term deal, but according to contracting documents, that amble was overturned because of a ruling that more market research needs to subsist conducted to support a conclusion to warrant a sole source award.
The agency touted IBM's rating in a recent performance assessment.
"IBM's performance is solid and continues to ameliorate over the course of this contract. They abide moved from the satisfactory/good even to the very good/excellent even in many areas. Their processes abide matured. Their knowledge groundwork continues to grow. IBM continues to ally with the government to overcome challenges and displays stalwart commitment to program success," the document stated.
GSA is in the midst of a major modernization of the SAM ecosystem, which touches every aspect of civilian and defense contracting, from solicitation to tracking spending. The effort has been in progress since 2010, and the late pace of the project has generated some ire on Capitol Hill, most notably from Sen. Claire McCaskill (D-Mo.), who threatened to hold up confirmation of Denise Turner Roth to lead GSA in 2015 in order to obtain answers to questions about the cost and timing of the project.
In September 2017, GSA launched a beta site that will eventually encompass nine fully modernized systems, eliminating the need for standalone sites such as FedBizOpps, the Federal Procurement Data System and others. So far, just one legacy system -- the Catalog of Federal Domestic Assistance -- has been retired.
Once the modernized system moves from beta to production, GSA anticipates more opportunities, including multiple small traffic awards to subsist made for the operation, maintenance and enhancement of the recent system.
symptom Rockwell is a senior staff writer at FCW, whose beat focuses on acquisition, the Department of Homeland Security and the Department of Energy.
Before joining FCW, Rockwell was Washington correspondent for Government Security News, where he covered every bit of aspects of homeland security from IT to detection dogs and margin security. Over the terminal 25 years in Washington as a reporter, editor and correspondent, he has covered an increasingly wide array of high-tech issues for publications like Communications Week, Internet Week, Fiber Optics News, tele.com magazine and Wireless Week.
Rockwell received a Jesse H. Neal Award for his toil covering telecommunications issues, and is a graduate of James Madison University.
Click here for previous articles by Rockwell. Contact him at firstname.lastname@example.org or ensue him on Twitter at @MRockwell4.
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